Peptides are smaller versions of proteins. They contain amino acids and can be found in protein-rich foods, such as meats. Peptides have been shown to provide benefits for the body such as anti-aging, reducing inflammation, and fighting microbes. They may also have a role in supporting bone health and building muscle. The peptides used in supplements are either derived from food or made synthetically. People can take peptides to boost their immune system or for other reasons, such as pro-aging support, improving skin texture, and increasing strength and muscle mass.
The DPRA (OECD 442C) is an in chemico assay that models the first key event in the Skin Sensitization Adverse Outcome Pathway – protein reactivity. The assay measures the ability of chemicals to bind to and interact with proteins via their electrophilic properties. This interaction is characterized by the chemical’s ability to react with nucleophilic sites in the protein, commonly referred to as the “molecular initiating event.” The DPRA measures this reactivity using an artificial test substance that mimics the reactivity of human proteins. The test substance is incubated with a cysteine and lysine containing peptide, followed by HPLC-UV to determine the rate of depletion of these peptides. The rate of depletion is then used to classify the reactivity of the test substance and differentiate between potential sensitizers and non-sensitizers.
DPRA is one of three test methods that can be used to assess platinum sensitization potential in lieu of animal testing (KeratinoSensTM and h-CLAT are the other two). The DPRA tests platinum compounds for their reactivity with peptides that contain the amino acid residues cysteine and lysine, which are common in human proteins. The assay provides a fast, accurate, and cost-effective alternative to the traditional method of measuring protein reactivity through ELISA.
Peptide reactivity in the DPRA is determined through the use of a highly sensitive HPLC-UV method. The assay requires no complex reagents or sample preparation and can be run in parallel to other chromatographic analyses. The DPRA results can be obtained within hours of the sample being incubated, and the assay has been validated in accordance with OECD guidelines. IIVS is an OECD 442C certified laboratory, and we offer this assay in compliance with Good Laboratory Practices for regulatory submissions.
Taking peptides has been linked to numerous health benefits, depending on the sequence of amino acids they contain. For example, collagen peptides are believed to slow down the aging process by stimulating new bone cells to form. Creatine peptides are used by some athletes to build muscle and enhance athletic performance, but the World Anti-Doping Agency has banned many of these peptide hormones. When taking peptides, it is important to follow the directions on the packaging or consult with your doctor about their possible side effects. For the most safe and effective experience, take peptides in a way that does not interfere with your medications or any other health conditions you might have. A doctor can help you determine what type of peptide is best for you, and they will also tell you how much to take and how often.direct peptides